Fig. 3.
a Tissue extracts from rat brain (10 μg) and MCA (20 μg) were run on 10% SDS-PAGE gels and probed with Panx1 antibody in the absence and presence of the control peptide. b Tissue extracts from rat brain (10 μg) and MCA (2.5 μg) were run on 10% SDS-PAGE gels and probed with Panx2 antibody in the absence and presence of the control peptide. Each band disappeared when the primary antibody was incubated with the control peptide. Each membrane was stripped and reprobed for GAPDH. The molecular mass is indicated. Data are representative of 4 separate preparations.