Fig. 4.

Pannexin immunofluorescence in frozen cross-sections of rat MCA. a Panx1 fluorescence (red) showed weak staining in smooth muscle (arrow) and was undetected in endothelial cells. b Red fluorescence was absent in sections in which the control rabbit IgG was substituted for the primary antibody. c Panx2 fluorescence (red) was detected in both endothelial cells (bottom two arrows) and smooth muscle cells (top arrow). d Red fluorescence was absent in sections incubated with a control rabbit IgG. Green fluorescence is the autofluorescence of the internal elastic lamina that separates the endothelium from smooth muscle. Blue fluorescence identifies nuclei labeled with DAPI. Data are representative of 3 separate preparations. Scale bar = 20 μm. (Color only in online version).