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. 2012 Feb;91(2):299–309. doi: 10.1189/jlb.0611308

Figure 3. ICAM-1 is required for IFN-γ- and TNF-α-induced target cell lysis.

Figure 3.

(A) ICAM-1 expression on THP-1 cells (dotted line), THP-1 cells treated with IFN-γ (black line), or TNF-α (light, gray line) for 20 h. Isotype control (Iso. ctrl.) is shown in gray-shaded histograms. (B) The ICAM-1 expression on THP-1 cells was detected by FACS analysis at 0-, 1-, 2-, 3-, 4-, 6-, and 8-h time-points following the treatment with IFN-γ (left panel) or TNF-α (right panel). (C) IFN-γ and TNF-α enhanced the conjugation between NK and THP-1 cells. (D) Lysis of THP-1 cells by NK-92MI cells at an E:T ratio of 1:1 and 4:1 for 2 h in the presence of ICAM-1 blocking antibody or control IgG. (E and F) Coincubation of THP-1 cells with primary NK cells for 4 and 8 h increased ICAM-1 expression on THP-1 cells, and the ICAM-1 induction is reduced significantly in the presence of IFN-γ- and TNF-α-neutralizing antibodies. The same data were presented as a bar diagram (E) and histogram (F). The experiments were repeated for at least two times, and one representative result was shown. FL1-H, FL-1 height; MFI, mean fluorescence intensity.