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. 2012 Feb;91(2):299–309. doi: 10.1189/jlb.0611308

Figure 4. The expression of ICAM-1 correlates with susceptibility to NK lysis.

Figure 4.

(A) Knockdown of ICAM-1 on THP-1 cells with siRNA. TNF-α induced ICAM-1 expression on THP-1 cells transfected with control siRNA (dotted lines) or ICAM-1-specific siRNAs (black lines). Isotype control staining is shown in gray-shaded histograms. (B) ICAM-1 expression on empty vector-transfected (dotted line) or ICAM-1-transfected (black line) THP-1 cells. (C and D) Correlation between IFN-γ (C)- or TNF-α (D)-induced ICAM-1 expression on various siRNA-transfected THP-1 cells and their lysis by NK-92MI cells. (E) Overexpression of ICAM-1 is sufficient for THP-1 cells to sensitize to NK-92MI-mediated lysis. (F) TPCA-1 and BAY 11-7082 (BAY) inhibit TNF-α-induced sensitization of THP-1 cells to NK-92MI-mediated cytotoxicity. The experiments were repeated for at least two times, and one representative result was shown.