Skip to main content
. 2012 Feb;91(2):275–284. doi: 10.1189/jlb.0711356

Figure 2. Effects of Pseudomonas ExoS and pyocyanin on neutrophil histamine production and release.

Figure 2.

(A) Effects of a range of concentrations of recombinant ExoS in comparison with effects of incubating naïve mouse PMNs alone or with live PAO1 strain of P. aeruginosa at a 1:10 ratio of PMN:bacteria. (B) Effects of incubating purified pyocyanin in experiments of similar design. (A and B) Black and white bars, amounts of total and extracellularly released histamine, respectively. (C) Results of incubating neutrophils with a range of ratios of WT PAO1 or isogenic mutant PAO1 bacteria containing genetic deletion of the ExoS gene (ΔExoS). For data in all panels, values represent mean ± se; n = 3 versus corresponding PMN-alone group: *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.