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. 2012 Feb;91(2):259–265. doi: 10.1189/jlb.0511239

Figure 2. Neutrophil recruitment activity of CXCL8 variants.

Figure 2.

Total neutrophils recruited into the peritoneum are plotted for each of the variants at different doses. Data are presented as mean ± sem and are from 10 to 19 animals from a total of two to four independent experiments with four to six animals/group for each experiment. One-way ANOVA was used to determine statistical significance. The recruitment of the WT compared with the monomer and dimer is significant at all doses (P<0.01), except at 1 μg between WT and monomer. The recruitment of WT and monomer compared with the control was significant at all doses (P<0.01) and of the dimer compared with control was significant only at the 10 μg dosage. Inset: The R6K mutation renders CXCL8 completely inactive, and its recruitment at 10 μg dose is similar to that of control PBS, indicating that recruitment as a result of spurious contamination or bacterial byproducts can be ruled out.