Figure 2.

Effect of miniagrin on the expression of muscle myosin and total DNA content in engineered muscle constructs. A) Protein levels of fast myosin and total myosin were normalized in agrin-treated cells relative to control constructs in each cell isolation. β-Tubulin served as the loading control; n = 6 constructs/group, from 3 independent cell isolations. B) Total DNA content in agrin-treated and control constructs; n = 9 constructs/group, from 3 independent cell isolations. No significant difference was found between the groups.