Figure 5.
Effect of α-NETA on twitch and tetanus force in engineered muscle constructs without and with miniagrin treatment. NETA denotes α-NETA treatment, applied between d 0 and 10 of differentiation. A) Representative force traces of a single twitch and tetanus from the same isolation in control, α-NETA-treated, and α-NETA-treated constructs exposed to miniagrin. B, C) Twitch force amplitude At (B) and tetanus-to-twitch ratio TtR (C) are decreased by α-NETA in both control and agrin-treated constructs; n = 9 constructs/group, from 3 independent cell isolations. *P < 0.05.