Figure 7.

Effect of miniagrin and α-NETA on AChR clustering in engineered muscle constructs. A) Representative confocal images of α-bungarotoxin-labeled AChR clusters in tissue constructs under different treatments. Scale bars = 20 μm. B–E) Effect of treatments on the percentage of myotubes containing AChR clusters (B), the average number of AChR clusters per myotube (C), distribution of AChR cluster lengths (D), and mean length of AChR cluster (E). Note that the number of >40-μm clusters in control group is 0. n = 9 images/group, from 3 constructs/group (each from an independent cell isolation). *P < 0.05.