Figure 1.

Effect on protein palmitoylation and growth factor signaling in BAECs treated with HPHG and hearts of mice fed HFHS diet. A) Ras (total Ras antibody) and eNOS palmitoylation is lower in cardiac tissue from mice fed a HFHS diet for 4 mo compared with a chow diet, as detected using the hydroxylamine dependent biotin-switch assay (n=4). B) Ras (total Ras antibody) and eNOS palmitoylation is also decreased in BAECs treated with medium containing HPHG for 16 h compared with control medium (n=6). C) In animals fed an HFHS diet, there is a substantial loss in basal ERK phosphorylation compared with mice fed chow (n=4). D) In BAECs treated with HPHG, there is also decreased basal ERK phosphorylation compared with control medium-treated cells (n=6). E) Basal and VEGF-induced ERK phosphorylation that was attenuated by preincubation of BAECs with HPHG was prevented in cells that overexpressed wild-type HRas (n=6). All graphs have been normalized for protein loading. Error bars = se. *P < 0.05, **P < 0.01; Student's t test.