Figure 3.

Intracellular activity of HRas in treated BAECs. This was determined by analyzing the colocalization of RBD with HRas overexpressed (Xpress-tag antibody) in BAECs that had been stimulated for 5 min with VEGF before being fixed. A) In control medium-treated cells, there is some colocalization of HRas with RBD at the plasma membrane (indicated by the white arrows), which is also apparent in the fluorescence intensity scans generated along the white lines indicated for each analyzed cell. B) In HPHG-treated BAECs, there is less membrane colocalization and also no increase in RBD staining with the accumulated HRas at the Golgi as indicated in the intensity scan for each analyzed cell.