Figure 6.

Effect of ERK inhibition and blocking HRas plasma membrane signaling in BAECs on growth factor and apoptotic signaling. A) Inhibition of MEK by U0126 attenuated basal ERK phosphorylation and increased caspase-3 cleavage 16 h after treatment. B) Overexpression of inactive dominant negative N17-HRas (total Ras antibody) in BAECs attenuated VEGF-dependent ERK phosphorylation (n=3). C) Overexpression of N17-HRas also increased cell apoptotic signaling in control medium-treated BAECs and did not protect cells from the apoptotic effect of HPHG (n=3). All graphs have been normalized for protein loading. Error bars = se. *P < 0.05, **P < 0.01, ^#P < 0.005; Student's t test.