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LYVE-1 expression in corneal lymphatics during aging. A, B) Double staining of corneal flatmounts for vascular (CD31, green) and lymphatic endothelium (LYVE-1, red) in untreated 3-wk-old (A) and 6-mo-old (B) C57BL/6. C) Quantitative analysis of the number of LYVE-1− lymphatic gaps in untreated corneas of C57BL/6 mice (3 wk old, n=8; 8 wk old, n=5; 6 mo old, n=11). *P < 0.05.
