Figure 3.

Histopathological changes in bleomcyin-induced pulmonary fibrosis associated with the genetic removal of CDH11. A) Lungs were taken from mice 21 d after 3.5-U bleomycin installation and processed for sectioning and H&E staining (A). B, C) Collagen deposition (B) and myofibroblasts (C) in distal air-spaces were assessed with Masson's trichrome and α SMA immunohistochemistry. D, E) Fibrosis was quantified by determination of collagen present in BAL fluid (D) and Ashcroft scores (E) on H&E-stained lung sections. Scores were determined on 20 images/mouse lung. n = 6 (WT saline; Cdh11 ^−/− saline), n = 13 (WT bleomycin), and n = 11 (Cdh11 ^−/− bleomycin). Scale bars = 500 μm (A); 100 μm (B, C).*P < 0.05 vs. WT saline; ^#P < 0.05 vs. WT bleomycin.