Figure 6.

CDH11 is present on alveolar macrophages. A–C) Representative images of confocal microscopy on BAL cells stained with CDH11 (23C6) antibody isolated from patients with ILD (A), patients with IPF (B), and mice given bleomycin (C). Images representative of n = 2 (ILD; IPF). D) Cultured MHS alveolar macrophages stained with CDH11 (23C6) antibody. E) Cdh11 transcripts measured in MHS alveolar macrophages were stimulated with 10 μg/ml LPS. Data are representative of 5 separate experiments and are presented as mean ± se fold change vs. medium alone. F) Protein lysates from MHS cells and WT dermal fibroblasts demonstrate CDH11 expression by Western blot using anti-CDH11 monoclonal antibody (clone 5B2H5). Scale bars = 50 μm (A–C); 20 μm (D).