Figure 5.

Light responses of loss-of-function tax-2, tax-4, cng-1, and cng-3 mutant worms expressing (b)opsin in neurons. A, B) WT worms with neuronal transgenic expression of (b)opsin (circles; A, B) or worms with loss-of-function mutants tax-2 (diamonds; A), tax-4 (squares; A), cng-1 (diamonds; B) or cng-3 (squares; B) expressed in neurons were preincubated with 9-cis-retinal and tested for their responses to blue light (1 s, 1000 lux, 488±20 nm). Data were derived from 3 independent experiments with 5–11 animals each. C) WT worms and quadruple loss-of-function mutant of cng-1;cng-3;tax-2;tax-4 worms, both with pan-neuronal expression of (b)opsin, were tested for their response to blue light (1 s, 1000 lux, 488±20 nm). Data represent 3 independent experiments with 3–10 animals each. Error bars indicate means ± se. ***P < 0.001; 2-way ANOVA.