Figure 3.
Analysis of wild-type and mutated COL3A1 mRNA to measure the silencing effect of different siRNAs in human fibroblast tissue culture. A) Amounts of mutated (COL3A1G252V/+) and wild-type COL3A1 mRNA were analyzed by real-time RT-PCR in untransfected cells (mock), after transfection of a nonsilencing siRNA (siC) and a set of 19 siRNAs targeting mutated COL3A1 (si1–si19). Data are averages ± se of 2 biological replicates. Results were analyzed by the 2−ΔΔCT method normalized to GAPDH. Result of the nonsilencing control was set as 100%. B) Silencing factor was the result of the proportion of wild-type/mutated form of COL3A1 mRNA.