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Western blot analysis of COL3A1 in extracts of human fibroblast tissue culture. Extracts (15 μg) of normal donor (+/+) and patient with vEDS (G252V/+) fibroblasts treated with 100 nM siC (control) or si10 siRNA for 12 d were compared with nontreated fibroblasts. COL3A1 was detected with a rabbit polyclonal antibody. As loading control, β-actin was detected with a mouse monoclonal antibody.
