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. 2011 Dec 5;51(1):90–99. doi: 10.1021/bi201356v

Figure 2.

Figure 2

SSNMR shows effects of peptides on the membranes. 1H–13C CPMAS spectra of 1:1 POPC/cholesterol in presence of (a) 10 mol % Cav82–109 (unlabeled), (b) 10 mol % Cav83–102 (unlabeled), or (c) without peptide. Selected assignments of POPC and cholesterol are shown, the latter as italicized numbers (#; see inset). In presence of the peptides, 13C signals throughout the lipids are broadened and some previously missing mobile POPC signals become visible. Static 31P NMR of POPC without (d) or with (e) 10 mol % Cav82–109, and 1:1 POPC/cholesterol in absence of peptide (f) or in presence of (g) 10 mol % Cav83–102 or (h) 10 mol % Cav82–109 shows that the lipids predominantly form a bilayer, with less than 5% present as isotropic signals. Peptide incorporation changes the lipid headgroup dynamics, seen as a reduction of the bilayer 31P CSA (see dashed vertical lines). Measurements were done with full hydration, at 300 K and 600 MHz 1H frequency.