Table 3.
Accuracy and Precisiona
| QC1 | QC2 | QC3 | QC4 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| analytes | intraday | interday | intraday | interday | intraday | interday | intraday | interday | ||||||||
| accuracy | precision | accuracy | precision | accuracy | precision | accuracy | precision | accuracy | precision | accuracy | precision | accuracy | precision | accuracy | precision | |
| 6k PGF1a d4 | N.D.b | N.D. | N.D. | N.D. | 2.16% | 1.48% | 2.74% | 3.6% | 6.76% | 3.64% | 0.06% | 8% | 7.25% | 1.63% | 2.00% | 6% |
| 6k PGF1a | N.D. | N.D. | N.D. | N.D. | 15.94% | 7.84% | 7.94% | 3.1% | 0.21% | 3.31% | 4.94% | 7% | 2.50% | 3.38% | 0.50% | 6% |
| TXB2 | 18.33% | 9.78% | 9.92% | 13% | 2.69% | 4.32% | 8.54% | 15% | 1.41% | 3.52% | 4.46% | 12% | 6.54% | 4.28% | 0.54% | 12% |
| 9-12-13 TriHOME |
o.r.c | o.r. | o.r. | o.r. | 15.00% | 16.7% | 7.56% | 10.3% | 12.75% | 2.35% | 11.65% | 14% | 10.08% | 4.47% | 4.20% | 14% |
| 9-10-13 TriHOME |
o.r. | o.r. | o.r. | o.r. | 14.44% | 4.6% | 19.33% | 17.4% | 4.78% | 0.75% | 6.93% | 2.2% | 1.33% | 2.30% | 5.00% | 4% |
| PGF2a | 10.71% | 7.97% | 9.33% | 9.5% | 16.67% | 4.43% | 18.00% | 11% | 13.94% | 2.30% | 10.91% | 8% | 2.42% | 0.54% | 2.18% | 6% |
| PGE2-d4 | 7.12% | 14.98% | 16.23% | 7% | 1.33% | 2.64% | 3.53% | 10% | 4.00% | 2.43% | 12.27% | 5% | 10.00% | 5.15% | 4.93% | 6% |
| PGE2 | 10.67% | 8.18% | 9.303% | 6.2% | 7.66% | 8.45% | 11.27% | 17% | 9.56% | 2.10% | 1.73% | 11% | 11.11% | 4.89% | 1.87% | 7% |
| PGD2 | 17.7% | 11.71% | 12.16% | 11.5% | 13.6% | 9.54% | 18.36% | 17% | 17.14% | 5.50% | 6.43% | 8% | 11.31% | 10.28% | 3.07% | 5% |
| 11 12 15 THET | N.D. | N.D. | N.D. | N.D. | 15.90% | 8.18% | 8.82% | 16.2% | 6.41% | 5.83% | 10.46% | 14% | 15.90% | 3.46% | 2.15% | 2% |
| Lipoxin A4 | 16.72% | 10.33% | 11.61% | 15.5% | 7.22% | 11.35% | 11.33% | 13% | 6.85% | 0.34% | 5.33% | 17% | 11.85% | 2.55% | 2.00% | 1.7% |
| PGB2/PGJ2 | o.r. | o.r. | o.r. | o.r. | 12.04% | 4.35% | 11.35% | 14% | 5.31% | 4.38% | 8.37% | 5% | 2.93% | 3.27% | 5.27% | 4% |
| THF Diols | N.D. | N.D. | N.D. | N.D. | 18.33% | 11.95% | 18.36% | 17% | 1.85% | 8.89% | 2.55% | 7% | 3.03% | 1.08% | 0.73% | 9% |
| LTB4 | N.D. | N.D. | N.D. | N.D. | 12.78% | 12.61% | 11.44% | 13% | 2.96% | 2.01% | 1.33% | 10% | 0.19% | 1.70% | 0.56% | 8% |
| 12 13 DHOME | 15.00% | 3.64% | 9.61% | 11.5% | 15.00% | 3.11% | 11.44% | 14% | 14.44% | 2.91% | 13.33% | 7% | 3.33% | 2.34% | 4.67% | 6% |
| 10 11 DHHep | 12.97% | 10.95% | 7.74% | 7.2% | 5.24% | 12.15% | 10.71% | 14% | 4.40% | 1.20% | 13.14% | 8% | 3.93% | 2.44% | 2.36% | 9% |
| 9 10 DHOME | 17.04% | 4.76% | 11.78% | 17% | 13.33% | 2.92% | 16.89% | 11% | 17.04% | 0.91% | 15.67% | 11% | 1.30% | 6.02% | 4.78% | 10% |
| 14 15 DHET | 17.78% | 8.11% | 16.09% | 13% | 8.61% | 7.53% | 0.58% | 16% | 13.89% | 2.96% | 0.67% | 7% | 17.78% | 4.60% | 4.83% | 5% |
| 11 12 DHET | 15.83% | 7.76% | 13.83% | 4.9% | 12.78% | 5.64% | 1.17% | 14% | 8.33% | 3.28% | 8.50% | 7% | 6.39% | 4.48% | 2.83% | 10% |
| 8 9 DHET | 15.94% | 6.79% | 13.83% | 12% | 14.17% | 6.40% | 6.83% | 5% | 6.39% | 5.93% | 8.17% | 8% | 4.72% | 4.40% | 6.33% | 9% |
| 15 deoxy PGJ2 | 18.75% | 7.20% | 10.73% | 14% | 10.08% | 0.67% | 12.30% | 16% | 16.42% | 2.30% | 11.05% | 13% | 3.42% | 1.01% | 9.40% | 11% |
| 19 HETE | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 4.80% | 9.48% | 10.56% | 10% | 8.80% | 4.67% | 1.20% | 12% |
| 20 HETE d6 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 5.83% | 10.41% | 2.06% | 9% | 12.03% | 4.96% | 7.46% | 12% |
| 20 HETE | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 17.47% | 7.28% | 4.52% | 10% | 5.40% | 4.38% | 5.52% | 5% |
| 5 6 DHET | 10.00% | 5.12% | 9.93% | 8.7% | 4.72% | 6.43% | 6.83% | 11% | 1.67% | 5.15% | 9.58% | 10% | 3.61% | 2.78% | 1.83% | 10% |
| 13 HODE | 13.52% | 5.34% | 8.67% | 3% | 1.85% | 0.56% | 6.89% | 4.5% | 1.22% | 1.66% | 0.18% | 10% | 9.37% | 2.47% | 6.60% | 9% |
| 9 HODE d4 | N.D. | N.D. | N.D. | N.D. | 0.19% | 8.50% | 9.18% | 3.5% | 6.11% | 3.13% | 4.67% | 11% | 1.33% | 2.79% | 6.56% | 11% |
| 9 HODE | 14.81% | 2.48% | 5.89% | 4.2% | 3.44% | 1.63% | 6.22% | 7% | 1.96% | 0.92% | 3.60% | 14% | 10.63% | 0.50% | 5.04% | 9% |
| 10 11 DHN | 13.42% | 3.91% | 19.54% | 8.3% | 0.73% | 6.69% | 2.25% | 18% | 10.10% | 3.43% | 1.88% | 11% | 7.81% | 5.60% | 1.44% | 10% |
| 15 HETE | 18.89% | 10.18% | 15.67% | 13% | 9.11% | 2.50% | 2.80% | 8% | 2.11% | 1.86% | 2.10% | 12% | 8.72% | 4.66% | 2.70% | 10% |
| 13 oxo ODE | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 9.29% | 7.23% | 11.57% | 14% | 11.43% | 6.09% | 1.71% | 11% |
| 11 HETE | 10.56% | 9.81% | 12.52% | 9% | 17.78% | 4.65% | 12.50% | 9% | 12.56% | 4.36% | 15.30% | 10.3% | 8.89% | 10.92% | 8.63% | 5% |
| 9 oxo ODE | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | 1.67% | 4.26% | 3.29% | 11% | 14.76% | 4.22% | 9.57% | 10% |
| 12 HETE | 16.94% | 10.44% | 10.67% | 7% | 8.94% | 6.63% | 12.73% | 12% | 3.50% | 1.48% | 0.13% | 14% | 3.11% | 3.68% | 1.03% | 11% |
| 8 HETE | 13.89% | 8.80% | 10.08% | 11% | 11.17% | 10.57% | 9.20% | 10% | 1.56% | 3.77% | 0.57% | 14% | 7.00% | 2.48% | 1.83% | 12% |
| 9 HETE | 9.17% | 4.07% | 11.97% | 3% | 9.94% | 2.46% | 11.37% | 2% | 4.44% | 3.34% | 9.53% | 14% | 12.33% | 3.31% | 7.30% | 11% |
| 5 HETE d8 | 7.75% | 2.42% | 9.00% | 9% | 8.89% | 4.67% | 13.42% | 4% | 11.67% | 1.89% | 15.00% | 16% | 8.42% | 3.72% | 2.47% | 5% |
| 5 HETE | 10.83% | 4.49% | 8.83% | 6% | 9.83% | 7.75% | 11.00% | 4% | 3.50% | 2.25% | 10.43% | 14% | 14.72% | 2.22% | 11.00% | 10% |
| 12 13 EpOME | 11.67% | 3.68% | 9.45% | 14% | 8.50% | 5.69% | 10.50% | 6% | 6.17% | 1.63% | 12.50% | 10% | 5.67% | 2.92% | 8.90% | 9% |
| 14 15 EET | N.D. | N.D. | N.D. | N.D. | 12.00% | 17.24% | 6.75% | 8.1% | 10.38% | 1.81% | 8.10% | 11% | 2.54% | 4.77% | 5.28% | 12% |
| 9 10 EpOME | 10.83% | 5.96% | 7.50% | 9.2% | 9.33% | 2.62% | 9.60% | 13% | 3.00% | 1.86% | 8.90% | 13% | 5.33% | 4.27% | 4.50% | 7% |
| 11 12 EET d8 | 11.04% | 10.25% | 10.13% | 16% | 11.88% | 4.87% | 2.75% | 16% | 5.42% | 3.17% | 6.13% | 16% | 11.88% | 4.45% | 10.00% | 6% |
| 11 12 EET | 6.63% | 3.47% | 7.38% | 7.1% | 10.44% | 1.78% | 6.25% | 4% | 11.00% | 1.83% | 7.35% | 10% | 12.71% | 2.41% | 5.33% | 12% |
| 8 9 EET | 10.63% | 7.50% | 8.00% | 8.2% | 11.42% | 8.18% | 12.58% | 6.5% | 17.08% | 9.41% | 12.05% | 14% | 13.21% | 2.10% | 6.48% | 13% |
| 5 6 EET | N.D. | N.D. | N.D. | N.D. | o.r. | o.r. | o.r. | o.r. | 6.17% | 10.22% | 6.60% | 12% | 9.38% | 3.09% | 9.55% | 11% |
| 15 oxo ETE | 10.37% | 14.26% | 8.61% | 8% | 14.00% | 9.26% | 15.33% | 5% | 9.44% | 4.26% | 12.49% | 7% | 6.37% | 9.46% | 7.31% | 9% |
| 5 oxo ETE | N.D. | N.D. | N.D. | N.D. | 16.52% | 6.91% | 14.22% | 12.78% | 18.78% | 6.45% | 2.60% | 7.8% | 2.67% | 3.58% | 5.40% | 7% |
a
Accuracy represents the difference between the measured and expected value (n=3). Precision represents the relative standard deviation of the measurements (n=6).
b
N.D. the concentration is below of LOQ.
c
o.r. out of the linear range.