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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: J Biol Chem. 2007 Jul 1;282(34):24866–24872. doi: 10.1074/jbc.M703902200

Figure 1. FKBP mutants destabilize YFP expression.

Figure 1

A, The structure of FKBP F36V is shown as a ribbon diagram (PyMol) complexed with the SLF* ligand (PDB ID 1BL4). Three representative mutations are highlighted in red, and the ligand is shown in blue.

B, Fluorescence of FKBP-YFP and YFP-FKBP fusions expressed in NIH3T3 cells in the absence of stabilizing ligand as determined by analytical flow cytometry. Data are presented as the average mean fluorescence intensity (MFI) ± SEM standardized to the fusions between F36V and YFP.