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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: J Biol Chem. 2007 Jul 1;282(34):24866–24872. doi: 10.1074/jbc.M703902200

Figure 2. Synthetic FKBP ligands stabilize the V2A-YFP fusion protein.

Figure 2

A, Chemical structures of FKBP ligands.

B, NIH3T3 cells stably expressing the V2A-YFP fusion protein were treated with 3-fold dilutions of each FKBP ligand (10 μM to 300 nM). YFP expression levels were measured upon treatment with SLF* acid (diamonds), SLF*-N (squares), SLF*-T (triangles) and Shield-1 (circles) by flow cytometry.

C, NIH3T3 cells stably expressing the V2A-YFP fusion protein were treated with 3-fold dilutions of Shield-1 (10 μM to 1 nM) and assayed by flow cytometry. Data are presented as the average MFI ± SEM relative to that of the F36V FKBP protein. The dotted line represents the YFP expression level for V2A-YFP in the absence of Shield-1.