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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: J Biol Chem. 2007 Jul 1;282(34):24866–24872. doi: 10.1074/jbc.M703902200

Figure 3.

Figure 3

Shield-1 stabilizes the FKBP-YFP fusion proteins. NIH3T3 cells stably expressing the indicated fusion proteins were split into two populations that were either mock-treated (−) or treated with 1 μM Shield-1 (+) for 24 hours. FKBP mutants were fused to the N-terminus (panel A) or the C-terminus (panel B) of YFP, and expression levels were measured by flow cytometry and normalized to cells expressing the parent F36V-YFP protein treated with 1 μM Shield-1. Data present the average of three experiments ± SEM.