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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: J Biol Chem. 2007 Jul 1;282(34):24866–24872. doi: 10.1074/jbc.M703902200

Figure 4. Characterization of additional FKBP mutants.

Figure 4

A, FKBP-YFP fusions were either mock-treated (−) or treated with 1 μM Shield-1 (+) and YFP expression levels were monitored by flow cytometry. Data are presented as the average MFI ± SEM relative to that of the parent F36V FKBP.

B, NIH3T3 cells stably expressing FKBP-YFP fusion proteins were treated with 1 μM Shield-1 for 24 hr. The cells were then washed with media to remove Shield-1, and decreases in fluorescence intensity were monitored by flow cytometry: V2A-YFP (squares), V4A-YFP (triangles), V2A/I7V-YFP (diamonds) and V4A/I7V-YFP (circles).