Table 1.
Effects of Shield-1 on Gene Expression in NIH3T3 Cells
| Upregulated Genes | |||||
|---|---|---|---|---|---|
| Clone ID | Gene Symbol | Gene description | Fold Change (FDR*) | ||
| 10 nM | 100 nM | 1 μM | |||
| mSQ030922 | Epb4.1l2 | Erythrocyte protein band 4.1-like 2 | 2.2 (<0.1%) | ||
| mSQ001430 | Csnk1e | Casein kinase 1, epsilon | 2.7 (<0.1%) | ||
| mSQ002857 | Tcfe3 | Transcription factor E3 | 1.9 (27.5%) | ||
| Downregulated Genes | |||||
|---|---|---|---|---|---|
| Clone ID | Gene Symbol | Gene description | Fold Change (FDR*) | ||
| 10 nM | 100 nM | 1 μM | |||
| mSQ036549 | MtCrVgp3 | Murine type C retrovirus | −11.3 (<0.1%) | ||
| mSQ009285 | 4930422M22Rik | RIKEN cDNA 4930422M22 gene | −2.2 (<0.1%) | ||
| mSQ007437 | A430110N23 | hypothetical protein A430110N23 | −2.1 (<0.1%) | ||
| mSQ014243 | Ptpn1 | Protein tyrosine phosphatase, non- receptor type 1 | −1.7 (<0.1%) | ||
| mSQ001772 | Npas4 | Neuronal PAS domain protein 4 | −1.7 (22.7%) | ||
| mSQ028092 | Shkbp1 | Sh3kbp1 binding protein 1 | −2.3 (22.7%) | ||
*
NIH3T3 cells in triplicate were cultured in media and mock-treated or treated with 10 nM, 100 nM or 1 μM Shield-1 for 24 hours. Total RNA from each of the twelve samples was extracted and hybridized to the MEEBO oligonucleotide array. The false discovery rate (FDR) was obtained using unpaired two-class Significance Analysis of Microarrays (SAM), and genes whose mRNA levels changed significantly upon Shield-1 treatment were identified.