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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: Cell. 2006 Sep 8;126(5):995–1004. doi: 10.1016/j.cell.2006.07.025

Figure 3. Fusion of an FKBP Destabilizing Domain to the N Terminus of YFP Results in Predictable and Reversible Small-Molecule Regulation of Intracellular Protein Levels.

Figure 3

A population of NIH3T3 cells stably expressing L106P-YFP was treated with varying concentrations of Shld1 over the course of one week, and samples of the population were assayed by flow cytometry at the indicated time points. Data are presented as the average mean fluorescence intensity ± SEM relative to that of the maximum fluorescence intensity observed for L106P-YFP. Predicted fluorescence is based upon the dose response experiment shown in Figure 2B. The experiment was performed in triplicate.