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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: Cell. 2006 Sep 8;126(5):995–1004. doi: 10.1016/j.cell.2006.07.025

Figure 4. FKBP Destabilizing Domains Confer Shld1-Dependent Stability to a Variety of Proteins.

Figure 4

(A) FKBP mutants F15S and L106P were fused to the N termini of several different proteins and transduced into NIH3T3 cells. Cell populations stably expressing the fusions were then either mock-treated or treated with 1 mM Shld1, and cell lysates were immunoblotted with antibodies against the protein of interest. Endogenous proteins are shown as loading controls when detected, and Hsp90 serves this purpose in cases where they are not detected.

(B) FKBP mutants D100G and L106P were fused to the C termini of several different proteins of interest and treated as above.