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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: Cell. 2006 Sep 8;126(5):995–1004. doi: 10.1016/j.cell.2006.07.025

Figure 5. Destabilizing Domains Confer Shld1-Dependent Stability to a Transmembrane Protein.

Figure 5

FKBP mutants D100G and L106P were fused to the C terminus of CD8α, and NIH3T3 cells stably expressing the fusions were split into three pools. The first population (−) was mock-treated and the second population (+) was treated with 1 mM Shld1 for 24 hr. The third population (+/−) was treated with 1 mM Shld1 for 24 hr, then washed with media and cultured for 24 hr in the absence of Shld1. Live cells were then probed with a FITC-conjugated anti-CD8a antibody and assayed by flow cytometry. Data are presented as the average mean fluorescence intensity ± SEM from an experiment performed in triplicate.