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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: Cell. 2006 Sep 8;126(5):995–1004. doi: 10.1016/j.cell.2006.07.025

Figure 6. Stabilization of Specific Proteins with Shld1 Results in Predictable Changes in Cellular Morphologies.

Figure 6

NIH3T3 cells stably expressing fusions of a constitutively active small GTPase to the L106P destabilizing domain were split into three pools. The first population (−) was mock-treated and the second population (+) was treated with 1 mM Shld1 for 24 hr. The third population (+/−) was treated with 1 mM Shld1 for 24 hr, then washed with media and cultured in the absence of Shld1 for 24 hr (RhoA Q63L) or 48 hr (Cdc42 Q61L, Arl7 Q72L). Cells were serum-starved for 12 hr, fixed, stained with Alexa Fluor 488-conjugated phalloidin, and visualized using confocal microscopy.