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. Author manuscript; available in PMC: 2012 Feb 29.
Published in final edited form as: J Mol Cell Cardiol. 2009 Aug 28;47(6):835–845. doi: 10.1016/j.yjmcc.2009.08.017

Fig. 6. Terminal Deoxynucleotidyl Transferase-Mediated dUTP Nick-End Labeling (TUNEL) and Western blot analysis.

Fig. 6

a, In situ cell death detection kit was used for the detection of apoptotic cells. Three independent experiments were performed. TUNEL-positive cells were visualized using a confocal microscope. b, An example of a TUNEL-positive myocyte (red) was shown with cardiac myocyte membranes (green) and nuclei (blue). The fraction of apoptotic cells was determined by dividing the number of TUNEL-positive cells (red) by the total number of DAPI-positive cardiac myocyte nuclei (blue). c, Immunoblots showing sEH protein from LV free wall in four different groups of animals; treated and untreated sham and MI. GAPDH protein was used as an internal loading control. d, Summary data showing the densitometry of sEH protein level normalized to GAPDH level in the four groups of animals.