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. 2012 Feb 29;7(2):e32531. doi: 10.1371/journal.pone.0032531

Figure 1. Intracellular distribution of Cx31 variants in stable cell lines.

Figure 1

Hela cells stably expressing Cx31WT, Cx31R42P and their control line (Hela) were grown either at 37°C or at 26°C. Expression of Cx31 variants (green) and cell nuclei (blue) are shown. High magnification pictures of Cx31R42P-expressing cells are also shown to exemplify the gap junction plaque-like structures and small condensed nuclei (bottom panel). Note that Cx31WT-EGFP form gap junction plaques between adjacent cells incubated at 37°C (B). At 26°C, these structures are enlongated (E). Cx31R42P is barely detectable at 37°C (C). It is accumulated predominantly in cytoplasm (F, G) and can form gap junctions (F and G, red arrows) at 26°C. Small condensed nuclei (SN) are observed in Cx31R42P-expressing cells cultured at 26°C (F, H and I; white arrows). Bar = 20 µm.