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. 2012 Feb 29;7(2):e32531. doi: 10.1371/journal.pone.0032531

Figure 4. Cx31R42P induced ER stress promotes ROS production.

Figure 4

(A) Bip expression in Hela, Cx31WT (WT) and Cx31R42P (R42P) cells incubated at 37°C or 26°C (upper panel). Expression of Cx31 variants (middle panel) and β-actin (lower panel) is shown. (B) Quantification of Cx31R42P cells with SN after salubrinal treatment (10 µM) or control solvent (DMSO) treatment. (C) The rate of LDH release in Cx31R42P cells with either salubrinal treatment (10 µM) or control solvent (DMSO) treatment. (D–E) Relative level of intracellular ROS in control Hela cells, cells stably expressing Cx31WT (WT) and Cx31R42P (R42P) when they are cultured at 37°C (D) or 26°C (E). (F) Relative ROS level in Cx31R42P cells with either salubrinal (10 µM) or control solvent (DMSO) treatment. Error bars represent SEM. One star: P<0.05; two stars: P<0.01; three stars: P<0.001.