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. 2012 Feb 29;7(2):e32524. doi: 10.1371/journal.pone.0032524

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Aldrich et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Confocal immunofluorescence microphotographs of transgenic midgut sporozoites incubated at room temperature and developed with either a monoclonal antibody directed against the PbCSP repeat region (Pb-RR), a serum directed against the PgCSP N-terminal region (Pg-Nt), or a serum directed against the PgCSP repeat region (Pg-RR). Antibodies against the PbCSP and PgCSP repeat regions showed surface expression of the CSPs, whereas antibody against the PgCSP N-terminal region revealed mainly an intracellular pattern of expression.