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. 2012 Mar 1;23(5):758–770. doi: 10.1091/mbc.E11-08-0663

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© 2012 Chiang et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — P23H rhodopsin protein degradation depends on proteasome function. (A) P23H rhodopsin was expressed in cells bearing IRE1[I642G], and lactacystin (1 μM) and/or 1NM-PP1 (5 μM) was added for 24 h as indicated. Rhodopsin protein levels were detected by immunoblotting and quantified. (B) P23H rhodopsin was expressed in cells bearing IRE1[I642G]. MG132 (1 μM) and/or 1NM-PP1 (5 μM) were applied for 24 h as indicated. (A, B) GAPDH protein levels were assessed as a protein loading control. Immunoblots are representative of three independent experiments. Error bars represent SDs from three experiments. The p values were determined by Student's t test analysis.