FIGURE 7:

(A) Wild-type or mutant T17M, Y178C, D190G, K296E, or C185R rhodopsin was expressed in cells bearing IRE1[I642G], and 1NM-PP1 (5 μM) was applied for 24 h. Total rhodopsin protein levels were detected by immunoblotting and quantified. Error bars represent SDs from three experiments. The p values were determined by Student's t test analysis. *p < 0.05, **p < 0.01, ***p < 0.001. (B) CFTR∆F508 was expressed in cells bearing IRE1[I642G], and 1NM-PP1 (5 μM) was applied for 24 h. CFTR∆F508 protein levels were detected by immunoblotting and quantified. (A, B) GAPDH levels were assessed as a protein loading control.