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. 2012 Mar 1;23(5):781–791. doi: 10.1091/mbc.E11-06-0537

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© 2012 Leight et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — TGF-β1–induced EMT and apoptosis in MDCK cells cultured on polyacrylamide gels. (A) Immunostaining on rigid (5 kPa) and compliant (0.4 kPa) PA gels in cells treated with TGF-β1 or BSA control. (B) Graph of percentage of cells positive for cleaved caspase-3 immunofluorescence. n = 3 ± SEM. ^#p < 0.01 as compared with BSA conditions; ^§p < 0.01 as compared with 5- and 60-kPa TGF-β1 conditions. Bars, 50 μm.