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. 2012 Mar 1;23(5):781–791. doi: 10.1091/mbc.E11-06-0537

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© 2012 Leight et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Decreased matrix rigidity inhibits EMT independent of apoptosis. (A) Phase contrast and immunostaining for N-cadherin, E-cadherin, α-SMA, and nuclei of NMuMG cells infected with retro-Bcl-xL on compliant gels. (B) Western blot and quantification of N-cadherin, E-cadherin, α-SMA, and GAPDH in NMuMG cells infected with retro-GFP or retro-Bcl-xL or treated with 400 μM ZVAD-FMK, plated on rigid and compliant gels, and treated with TGF-β1. n = 4 ± SEM. Bars, 50 μm.