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. 2012 Mar 1;23(5):781–791. doi: 10.1091/mbc.E11-06-0537

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© 2012 Leight et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Effect of matrix rigidity and TGF-β1 on the actin cytoskeleton and focal adhesion formation in NMuMG cells. (A,B) Immunofluorescence images of F-actin (green), nuclei (blue), vinculin (magenta), and phospho-FAK (yellow) on rigid (8 kPa) (A) and compliant (0.4 kPa) (B) gels. Inset shows magnification of vinculin (V), phospho-FAK (F), and merged (M) images. (C) Western blot and quantification of phospho-FAK (125 kDa), total FAK (125 kDa), and GAPDH (38 kDa). Caspase-3 activity (D) and Snai1 mRNA expression (E) in NMuMG cells infected with Ad-GFP or Ad-FAK cultured on rigid and compliant gels treated with TGF-β1. n = 4 ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. Bars, 25 μm.