FIGURE 7:

Regulation of Akt activity by matrix rigidity in NMuMG cells. (A) Western blot and quantification of phospho-Akt (60 kDa), total Akt (60 kDa), and GAPDH (38 kDa) in cells plated on compliant and rigid polyacrylamide gels. (B–D) Caspase-3 activity and Snai1 mRNA expression in cells treated with DMSO control, 10 μM LY294002, 1 μM Akt inhibitor VIII, or 10 μM Akt inhibitor V. (E–G) Caspase-3 activity and Snai1 mRNA expression in cells infected with Ad-GFP or Ad-p110-CAAX. n = 3 ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ^§p < 0.01 rigid + insulin compared with BSA and TGF-β1 conditions; ^#p < 0.01 rigid + insulin/TGF-β1 compared with all conditions; ^φp < 0.01 compliant + insulin/TGF-β1 compared with compliant BSA condition.