FIGURE 5:

Inhibition of FA oxidation impairs cellular antioxidant defense. (A) Total glutathione (left) and GSSG (right) were measured in lysates from cells treated with or without TM and 50 μg/ml ET for 4 h. Both species were measured as described in Materials and Methods. n = 3 independent plates. (B) Cells were treated for 8 h with vehicle, 100 μg/ml ET, or 5 μg/ml GW6471, or for 1 h with 1 mM H₂O₂. Protein carbonylation was detected in lysates by reaction with 2,4-dinitrophenylhydrazine (DNP) and detection by an anti-DNP antibody.