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. 2012 Mar 1;23(5):896–909. doi: 10.1091/mbc.E11-09-0785

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© 2012 Velikkakath et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Autophagy-related proteins accumulate in Atg2A/B-depleted cells. HeLa cells or HeLa cells stably expressing the indicated GFP-fused proteins were treated with control siRNA or siRNA against Atg2A and Atg2B. Cells cultured in regular medium were fixed and stained with the anti-Atg9A, anti-LC3 (CTB-LC3-2-1C; Cosmo Bio), and anti-GFP (A6455; Invitrogen) antibodies. Immunofluorescence images were obtained using a confocal microscope. Signal color is indicated by color of typeface. Scale bar, 5 μm.