FIGURE 4:

Specific translation activities for Venus-ARC and Venus-FMRP RNA granules in wild-type and Fmr1 KO neurons. (A) Fluorescence intensities for individual well-resolved granules containing Venus-ARC RNA. (B) Fluorescence intensities for individual RNA molecules immobilized on glass coverslips, imaged under the same conditions as in A. (C–E) Specific translation activities for granules containing Venus-ARC RNA in wild-type neurons, Venus-FMRP RNA in wild-type neurons, and Venus-ARC in Fmr1 KO neurons are plotted with number of RNA molecules per granule (determined by comparing the total fluorescence intensity of the granule with the average intensity of individual RNA molecules) on the x-axis and number of events per granule per minute on the y-axis. The number of events per granule was determined by counting the number of centroids corresponding to newly synthesized Venus fusion proteins that appeared in the vicinity of each well-resolved granule over a period of 10 min.