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. 2012 Mar 1;23(5):945–954. doi: 10.1091/mbc.E11-08-0664

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© 2012 Naye et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — bmp2a is expressed in the LPM adjacent to the hepatopancreatic endoderm at 22 hpf. (A) Lateral view showing bmp2a expression in the LPM starting at 22 hpf. The square shows a close-up of the region indicated by the dotted lines. (B) bmp2a (green) and pdx1 (red) expression analyzed by fluorescence whole-mount in situ hybridization at 22 hpf. The transverse sections were analyzed by confocal microscopy. (C) bmp2a (green) and hhex (red) expression analyzed at 24 hpf. (D) bmp2a (green) and bmp2b (red) expression comparison at 24 hpf. The images of transverse sections are flat stacking of several consecutive optical sections. TO-PRO-3 (blue) labels nuclei. Yellow and white dots delineate the LPM and the notochord (nc), respectively.