Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 1;23(5):955–964. doi: 10.1091/mbc.E11-12-0995

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Lajoie et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 5: — Kar2p availability reveals changes in the ER misfolded protein during adaptation. (A) Wild-type cells expressing the fluorescent splicing reporter (SR) consisting of GFP replacing the HAC1 open reading frame produce a fluorescence signal only when spliced by ire1. SR-GFP–expressing cells were treated with 1 μg/ml Tm, and GFP signal was measured over time using flow cytometry. (B) Mobility of Kar2p-sfGFP untreated cells or cells treated with 1 μg/ml Tm for 4 or 16 h was measured by FRAP. Data were normalized to the mean D values of untreated cells for both time points. At 4 h, Tm induces significant decrease in Kar2p-sfGFP mobility. Once stress is resolved and folding capacity of the ER is restored, the Kar2p-sfGFP mobility returned to the unstressed D values.