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. 2012 Feb 29;7(2):e32368. doi: 10.1371/journal.pone.0032368

Figure 1. Expression of NDRG2 in breast cancer cell lines and the effect of Ad-NDRG2.

Figure 1

(A) T-47D, MCF-7, BCAP-37, HBL-100, ZR-751, SK-BR-3 and MDA-MB-231 cells were collected for extraction of protein and RNA and analyzed for NDRG2 expression using western blot and RT-PCR. (B) NDRG2 low-expression MCF-7 breast cancer cells were infected by adenovirus carrying NDRG2 (Ad-NDRG2) or negative control LacZ (Ad-LacZ). Thereafter, protein and RNA from these cells were extracted and subjected to western blot and RT-PCR analysis. β-actin was used as a loading control. The data are representative of three independent experiments.