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. 2012 Feb 29;7(2):e32368. doi: 10.1371/journal.pone.0032368

Figure 2. Effect of MCF-7 preconditioned media on HUVEC proliferation.

Figure 2

(A) HUVECs were grown to confluence and were then cultured in preconditioned media (derived from MCF-7 and Ad-LacZ-MCF-7 cells pretreated under normoxia and hypoxia for 24 or 48 h) for 24 h, and serum-free DMEM was used as a control. (B) HUVECs were grown to confluence and were then cultured in preconditioned media (derived from Ad-LacZ-MCF-7 or Ad-NDRG2-MCF-7 cells pretreated under normoxia and hypoxia for 24 or 48 h) for 24 h. Ad-LacZ -MCF-7(N) or Ad-LacZ-MCF-7 (H) was used as controls. N, normoxia; H, hypoxia. The data are the mean ± standard deviation (SD) for three independent experiments. Statistical significance was assessed using one-way ANOVA and Student's t-test. * or ** indicates P<0.05 or P<0.01, respectively, when compared with the control.