Skip to main content
. 2012 Feb 29;7(2):e32368. doi: 10.1371/journal.pone.0032368

Figure 3. Effect of MCF-7 preconditioned media on HUVEC tube formation and invasion.

Figure 3

(A and B) The tube formation assay was performed as described in Materials and Methodss. HUVECs were plated on Matrigel and incubated under hypoxia for 6 h. Tube lengths were measured with Image-Pro Plus software; the histograms represent the quantification of the tube length of HUVECs. (C and D) The invasion assay was performed as described in Materials and Methods. After 4 h under hypoxia, the amount of invaded cells was calculated in five random high-power fields (400×), and the number from the control group was used as a control. The histogram represents the quantification of cells that invaded. The data are the mean ± standard deviation (SD) for three independent experiments. Statistical significance was assessed using one-way ANOVA and Student's t-test. * or ** indicates P<0.05 or P<0.01, respectively, when compared with the control. The Ad-NDRG2 panel of Figure 3A is excluded from this article's CC-BY license. See the accompanying retraction notice for more information.