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. 2011 Oct 5;86(2):37. doi: 10.1095/biolreprod.111.095208

FIG. 7.

FIG. 7.

The ovarian surface epithelium gives rise to new granulosa cells during the follicle assembly period. A) Ovaries from P1, P3, and P7 pups were labeled with the cytoplasmic MitoTracker dye (MTO, magenta), cultured for 24–96 h, then fixed and stained with an antibody against FOXL2 (green, nuclear). Only the surface epithelium was labeled after 24 h, but obvious ingression of labeled cells was observed after 72–96 h in cultures started at P1 or P3, but not P7. Arrowheads point to FOXL2-positive cells labeled with MitoTracker. B) A few squamous MitoTracker-labeled FOXL2-positive cells (arrowheads) were incorporated into primordial follicles after 96 h in ovaries placed into culture at P3. Nuclei (blue) were stained with syto13. Original magnification ×20 (A) or ×40 (B); bars = 20 μm.