CASP3 protein immunolocalization (100×) and quantification by morphometric analysis. Fluorescence intensity of CASP3 was greater in (A) Ala-TIMP1-treated rats compared with (B) rTIMP1-treated rats. However, there was not a difference between PBS-treated rats and Ala-TIMP1-treated rats or rTIMP1-treated rats (C). Theca (t), granulosa (g), and ovarian cortex (c) are marked. The bottom images in each panel show nuclear staining (DAPI, blue) and a negative control using IgG, as marked. Original magnification ×100. D) Quantitative analysis shown in graph (ANOVA: P = 0.024).