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. 2011 Nov 2;86(2):47. doi: 10.1095/biolreprod.111.094680

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© 2012 by the Society for the Study of Reproduction, Inc.

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FIG. 4. — CASP3 protein immunolocalization (100×) and quantification by morphometric analysis. Fluorescence intensity of CASP3 was greater in (A) Ala-TIMP1-treated rats compared with (B) rTIMP1-treated rats. However, there was not a difference between PBS-treated rats and Ala-TIMP1-treated rats or rTIMP1-treated rats (C). Theca (t), granulosa (g), and ovarian cortex (c) are marked. The bottom images in each panel show nuclear staining (DAPI, blue) and a negative control using IgG, as marked. Original magnification ×100. D) Quantitative analysis shown in graph (ANOVA: P = 0.024).