Figure 3. Resonance frequency shift following sandwich hybridization assay with the complementary probe-PCA3 target.
PCA3 target was used in a sandwich hybridization assay with the complementary ASO combination. Upper Left panel shows a scanning electron micrograph of a single clean RhNW before hybridization. Upper Right panel shows the same RhNW after the sandwich hybridization assay, with a substantial number of 50 nm AuNPs bound. Bottom panel shows a significant leftward shift (~200 kHz lower frequency) of the resonance peak upon particle binding.